Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar.
Identifieur interne : 000036 ( Main/Exploration ); précédent : 000035; suivant : 000037Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar.
Auteurs : Christopher T. Straub [États-Unis] ; Ryan G. Bing [États-Unis] ; Jack P. Wang [États-Unis] ; Vincent L. Chiang [États-Unis] ; Michael W W. Adams [États-Unis] ; Robert M. Kelly [États-Unis]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2020.
Abstract
Background
Biological conversion of lignocellulosic biomass is significantly hindered by feedstock recalcitrance, which is typically assessed through an enzymatic digestion assay, often preceded by a thermal and/or chemical pretreatment. Here, we assay 17 lines of unpretreated transgenic black cottonwood (
Results
Of the 17 transgenic poplar lines examined with
Conclusions
Feedstock recalcitrance can be significantly reduced with transgenic plants, but finding the correct modification may require a large sample set to identify the most advantageous genetic modifications for the feedstock. Utilizing
DOI: 10.1186/s13068-020-01675-2
PubMed: 32180826
PubMed Central: PMC7065347
Affiliations:
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Adams</name><affiliation wicri:level="2"><nlm:affiliation>3Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>3Department of Biochemistry and Molecular Biology, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Kelly, Robert M" sort="Kelly, Robert M" uniqKey="Kelly R" first="Robert M" last="Kelly">Robert M. 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Straub</name><affiliation wicri:level="2"><nlm:affiliation>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh</wicri:cityArea></affiliation></author><author><name sortKey="Bing, Ryan G" sort="Bing, Ryan G" uniqKey="Bing R" first="Ryan G" last="Bing">Ryan G. Bing</name><affiliation wicri:level="2"><nlm:affiliation>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh</wicri:cityArea></affiliation></author><author><name sortKey="Wang, Jack P" sort="Wang, Jack P" uniqKey="Wang J" first="Jack P" last="Wang">Jack P. Wang</name><affiliation wicri:level="2"><nlm:affiliation>2Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>2Department of Forestry and Environmental Resources, North Carolina State University, Raleigh</wicri:cityArea></affiliation></author><author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><affiliation wicri:level="2"><nlm:affiliation>2Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>2Department of Forestry and Environmental Resources, North Carolina State University, Raleigh</wicri:cityArea></affiliation></author><author><name sortKey="Adams, Michael W W" sort="Adams, Michael W W" uniqKey="Adams M" first="Michael W W" last="Adams">Michael W W. Adams</name><affiliation wicri:level="2"><nlm:affiliation>3Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>3Department of Biochemistry and Molecular Biology, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Kelly, Robert M" sort="Kelly, Robert M" uniqKey="Kelly R" first="Robert M" last="Kelly">Robert M. Kelly</name><affiliation wicri:level="2"><nlm:affiliation>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh</wicri:cityArea></affiliation></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background</b></p><p>Biological conversion of lignocellulosic biomass is significantly hindered by feedstock recalcitrance, which is typically assessed through an enzymatic digestion assay, often preceded by a thermal and/or chemical pretreatment. Here, we assay 17 lines of unpretreated transgenic black cottonwood (</p></div><div type="abstract" xml:lang="en"><p><b>Results</b></p><p>Of the 17 transgenic poplar lines examined with </p></div><div type="abstract" xml:lang="en"><p><b>Conclusions</b></p><p>Feedstock recalcitrance can be significantly reduced with transgenic plants, but finding the correct modification may require a large sample set to identify the most advantageous genetic modifications for the feedstock. Utilizing </p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32180826</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Use of the lignocellulose-degrading bacterium <i>Caldicellulosiruptor bescii</i> to assess recalcitrance and conversion of wild-type and transgenic poplar.</ArticleTitle><Pagination><MedlinePgn>43</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-020-01675-2</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Biological conversion of lignocellulosic biomass is significantly hindered by feedstock recalcitrance, which is typically assessed through an enzymatic digestion assay, often preceded by a thermal and/or chemical pretreatment. Here, we assay 17 lines of unpretreated transgenic black cottonwood (<i>Populus trichocarpa</i>) utilizing a lignocellulose-degrading, metabolically engineered bacterium, <i>Caldicellulosiruptor bescii</i>. The poplar lines were assessed by incubation with an engineered <i>C. bescii</i> strain that solubilized and converted the hexose and pentose carbohydrates to ethanol and acetate. The resulting fermentation titer and biomass solubilization were then utilized as a measure of biomass recalcitrance and compared to data previously reported on the transgenic poplar samples.</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">Of the 17 transgenic poplar lines examined with <i>C. bescii</i>, a wide variation in solubilization and fermentation titer was observed. While the wild type poplar control demonstrated relatively high recalcitrance with a total solubilization of only 20% and a fermentation titer of 7.3 mM, the transgenic lines resulted in solubilization ranging from 15 to 79% and fermentation titers from 6.8 to 29.6 mM. Additionally, a strong inverse correlation (<i>R</i><sup>2</sup> = 0.8) between conversion efficiency and lignin content was observed with lower lignin samples more easily converted and solubilized by <i>C. bescii</i>.</AbstractText><AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">Feedstock recalcitrance can be significantly reduced with transgenic plants, but finding the correct modification may require a large sample set to identify the most advantageous genetic modifications for the feedstock. Utilizing <i>C. bescii</i> as a screening assay for recalcitrance, poplar lines with down-regulation of coumarate 3-hydroxylase 3 (C3H3) resulted in the highest degrees of solubilization and conversion by <i>C. bescii</i>. One such line, with a growth phenotype similar to the wild-type, generated more than three times the fermentation products of the wild-type poplar control, suggesting that excellent digestibility can be achieved without compromising fitness of the tree.</AbstractText><CopyrightInformation>© The Author(s) 2020.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Straub</LastName><ForeName>Christopher T</ForeName><Initials>CT</Initials><AffiliationInfo><Affiliation>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.</Affiliation><Identifier Source="GRID">grid.40803.3f</Identifier><Identifier Source="ISNI">0000 0001 2173 6074</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bing</LastName><ForeName>Ryan G</ForeName><Initials>RG</Initials><AffiliationInfo><Affiliation>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.</Affiliation><Identifier Source="GRID">grid.40803.3f</Identifier><Identifier Source="ISNI">0000 0001 2173 6074</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jack P</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>2Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695 USA.</Affiliation><Identifier Source="GRID">grid.40803.3f</Identifier><Identifier Source="ISNI">0000 0001 2173 6074</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>Vincent L</ForeName><Initials>VL</Initials><AffiliationInfo><Affiliation>2Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC 27695 USA.</Affiliation><Identifier Source="GRID">grid.40803.3f</Identifier><Identifier Source="ISNI">0000 0001 2173 6074</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Adams</LastName><ForeName>Michael W W</ForeName><Initials>MWW</Initials><AffiliationInfo><Affiliation>3Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.</Affiliation><Identifier Source="GRID">grid.213876.9</Identifier><Identifier Source="ISNI">0000 0004 1936 738X</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kelly</LastName><ForeName>Robert M</ForeName><Initials>RM</Initials><Identifier Source="ORCID">0000-0002-0639-3592</Identifier><AffiliationInfo><Affiliation>1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.</Affiliation><Identifier Source="GRID">grid.40803.3f</Identifier><Identifier Source="ISNI">0000 0001 2173 6074</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol Biofuels</MedlineTA><NlmUniqueID>101316935</NlmUniqueID><ISSNLinking>1754-6834</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biofuel</Keyword><Keyword MajorTopicYN="N">Caldicellulosiruptor</Keyword><Keyword MajorTopicYN="N">Extreme thermophiles</Keyword><Keyword MajorTopicYN="N">Lignocellulose</Keyword><Keyword MajorTopicYN="N">Poplar</Keyword></KeywordList><CoiStatement>Competing interestsThe authors declare that they have no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>02</Month><Day>04</Day></PubMedPubDate><PubMedPubDate 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IdType="pubmed">27917379</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li><li>Géorgie (États-Unis)</li></region></list><tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Straub, Christopher T" sort="Straub, Christopher T" uniqKey="Straub C" first="Christopher T" last="Straub">Christopher T. Straub</name></region><name sortKey="Adams, Michael W W" sort="Adams, Michael W W" uniqKey="Adams M" first="Michael W W" last="Adams">Michael W W. Adams</name><name sortKey="Bing, Ryan G" sort="Bing, Ryan G" uniqKey="Bing R" first="Ryan G" last="Bing">Ryan G. Bing</name><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><name sortKey="Kelly, Robert M" sort="Kelly, Robert M" uniqKey="Kelly R" first="Robert M" last="Kelly">Robert M. Kelly</name><name sortKey="Wang, Jack P" sort="Wang, Jack P" uniqKey="Wang J" first="Jack P" last="Wang">Jack P. Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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